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Inducing Adherence in Suspension Cells


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#1 Yue

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Posted 02 July 2009 - 05:04 AM

Hello all,

I'm looking for an easy way to induce adherence of a Jurkat suspension cell line I'm currently running. Some protocols I've found have suggested incubation in serum-free media (~ 10 mins in a collagen-coated plasticware) prior to doing fixation, but I just wanted to get some thoughts. I need to find an easier way to induce adherence in these cells for translocation assays, and spinning everytime I need to do a wash step is time-consuming and tedious. Any thoughts are appreciated!

Edited by Yue, 02 July 2009 - 05:05 AM.


#2 youyou

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Posted 20 July 2009 - 06:30 AM

Hello all,

I'm looking for an easy way to induce adherence of a Jurkat suspension cell line I'm currently running. Some protocols I've found have suggested incubation in serum-free media (~ 10 mins in a collagen-coated plasticware) prior to doing fixation, but I just wanted to get some thoughts. I need to find an easier way to induce adherence in these cells for translocation assays, and spinning everytime I need to do a wash step is time-consuming and tedious. Any thoughts are appreciated!


Hi

could you please pass me some of the protocols or articles you have found ? i would be grateful , and have you tried using fibronectin or vitronectin coating of plasticware to adhere the suspension cells ? but attention it also means incubation in serum free medium .




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