1 reply to this topic

[Yue]

Hello all,

I'm looking for an easy way to induce adherence of a Jurkat suspension cell line I'm currently running. Some protocols I've found have suggested incubation in serum-free media (~ 10 mins in a collagen-coated plasticware) prior to doing fixation, but I just wanted to get some thoughts. I need to find an easier way to induce adherence in these cells for translocation assays, and spinning everytime I need to do a wash step is time-consuming and tedious. Any thoughts are appreciated!

Edited by Yue, 02 July 2009 - 05:05 AM.

[youyou]

Yue, on Jul 2 2009, 03:04 PM, said:

Hello all,

I'm looking for an easy way to induce adherence of a Jurkat suspension cell line I'm currently running. Some protocols I've found have suggested incubation in serum-free media (~ 10 mins in a collagen-coated plasticware) prior to doing fixation, but I just wanted to get some thoughts. I need to find an easier way to induce adherence in these cells for translocation assays, and spinning everytime I need to do a wash step is time-consuming and tedious. Any thoughts are appreciated!

Hi

could you please pass me some of the protocols or articles you have found ? i would be grateful , and have you tried using fibronectin or vitronectin coating of plasticware to adhere the suspension cells ? but attention it also means incubation in serum free medium .