Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Awakening PBMCs


  • Please log in to reply
1 reply to this topic

#1 Cstar1

Cstar1

    member

  • Members
  • Pip
  • 1 posts
0
Neutral

Posted 02 July 2009 - 02:04 AM

Hi

I am trying to recover PBMCs from liquid Nitrogen storage for antigen stimulation work.
Do I need to rest the cells overnight before adding the antigens into the cell culture?
Because I found some of the cells don't really response to the anti-CD3 (positive control).

Thanks

#2 Astarte Biologics

Astarte Biologics

    member

  • Active Members
  • Pip
  • 20 posts
2
Neutral

Posted 13 July 2009 - 11:42 AM

Hi

I am trying to recover PBMCs from liquid Nitrogen storage for antigen stimulation work.
Do I need to rest the cells overnight before adding the antigens into the cell culture?
Because I found some of the cells don't really response to the anti-CD3 (positive control).

Thanks

Hi,

I've had good luck adding antigens right after thawing. When you say that some cells don't respond to anti CD3, how are you measuring that? Is it that some vials of cells don't respond or are you measuring by CFSE and seeing a bright population? If you can't get any proliferation to anti CD3 there's a problem all right but it depends a bit on the details of your method.




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.