Isolated nuclei and trypan blue
Posted 02 July 2009 - 12:43 AM
Long time listener, first time caller!
I'm currently isolating nuclei from HeLa cells and many protocols say that they "confirm the nuclear isolation and purity using trypan blue". I have used trypan in viability assays in the past to count blue/pink cells, however, what should isolated nuclei look like under trypan and how would they look different from whole cells?
Posted 02 July 2009 - 04:14 PM
Posted 04 July 2009 - 09:42 AM
They will look just like whole cells, only a lot smaller. The trypan blue should still be excluded by intact membranes.
Thanks for that! I'll take a look ..........