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Isolated nuclei and trypan blue


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#1 squallweathered

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Posted 02 July 2009 - 12:43 AM

Hi all,

Long time listener, first time caller!

I'm currently isolating nuclei from HeLa cells and many protocols say that they "confirm the nuclear isolation and purity using trypan blue". I have used trypan in viability assays in the past to count blue/pink cells, however, what should isolated nuclei look like under trypan and how would they look different from whole cells?

Many thanks!

#2 bob1

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Posted 02 July 2009 - 04:14 PM

They will look just like whole cells, only a lot smaller. The trypan blue should still be excluded by intact membranes.

#3 squallweathered

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Posted 04 July 2009 - 09:42 AM

They will look just like whole cells, only a lot smaller. The trypan blue should still be excluded by intact membranes.


Thanks for that! I'll take a look ..........




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