2 replies to this topic

[squallweathered]

Hi all,

Long time listener, first time caller!

I'm currently isolating nuclei from HeLa cells and many protocols say that they "confirm the nuclear isolation and purity using trypan blue". I have used trypan in viability assays in the past to count blue/pink cells, however, what should isolated nuclei look like under trypan and how would they look different from whole cells?

Many thanks!

[bob1]

They will look just like whole cells, only a lot smaller.  The trypan blue should still be excluded by intact membranes.

[squallweathered]

bob1, on Jul 3 2009, 01:14 AM, said:

They will look just like whole cells, only a lot smaller.  The trypan blue should still be excluded by intact membranes.

Thanks for that! I'll take a look ..........