Hi all,
Long time listener, first time caller!
I'm currently isolating nuclei from HeLa cells and many protocols say that they "confirm the nuclear isolation and purity using trypan blue". I have used trypan in viability assays in the past to count blue/pink cells, however, what should isolated nuclei look like under trypan and how would they look different from whole cells?
Many thanks!
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#2
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Hmmm, I think it's working
Posted 02 July 2009 - 04:14 PM
They will look just like whole cells, only a lot smaller. The trypan blue should still be excluded by intact membranes.
#3
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Posted 04 July 2009 - 09:42 AM
bob1, on Jul 3 2009, 01:14 AM, said:
They will look just like whole cells, only a lot smaller. The trypan blue should still be excluded by intact membranes.
Thanks for that! I'll take a look ..........
