There's anyone have any experience with preparation of the following lysis buffer to dissolve protein pellets after Protein extraction with Trizol?
4% CHAPS (0.065 M)
1% DTT (0.064 M)
I've been struggling to get Urea and Thiourea dissolved in water. It just won't dissolve!
Anyone that has been through the pain, can get me any ideas/ tips?
Do you prepare the solution fresh each time? What volume usually?
Thanks a lot!
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Urea/Thiourea lysis buffer for Protein Solubilization after Trizol Extraction
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