I want to evaluate translocation of my protein from the cytosol to the membrane and other wise (in respond to stimulation).
the problem is that this is a membrane protein (6- trans domain membrane), so I assume that if I use any fractionation kit, because it membrane protein I will get the protein in the membrane fraction, even though it might translocate to the cytosole..
does anybody know or try how to deal with this problem?
separation of mambrane protein
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