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about the microRNA seqence database


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#1 ljchen

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Posted 30 June 2009 - 02:02 AM

Hi,

I would like to clone pri-miRNA and pre-miRNA sequence into the lentivirol vecter. But, I can't find any information about the pri-miRNA and pre-miRNA sequence database. Does anyone kindly provide me any useful information or website? I will be grateful for any help or suggestion from your help. Thank you very much!!

#2 miRNA man

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Posted 30 June 2009 - 05:15 AM

Most people will clone the miRNA plus ~100 bp 5' and 3' and this should work. There was a paper, I think in PNAS about a year or 2 ago, that used several computational methods to predict the pri-miRNA, but I can't find it right now. If I find it I'll send you a PM.

Edit: Ah-ha! Found the article, the PMID is 17965236.

Edited by miRNA man, 30 June 2009 - 05:19 AM.


#3 Jon Moulton

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Posted 30 June 2009 - 06:34 AM

miRBase at the Sanger Institute is a good source for miRNA sequence data.

http://microrna.sang...ces/index.shtml
Jon D. Moulton
Gene Tools, LLC
www.gene-tools.com

#4 ljchen

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Posted 03 July 2009 - 06:50 AM

Thank you very much for your suggestion!! :(




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