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tri-reagent rna isolation


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#1 wntiong

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Posted 30 June 2009 - 12:57 AM

hi, lets say if i want to improve my rna yield and purity, can i perform the extraction again using the extracted rna with 1:1 ratio of tri-reagent? or anyway will do to help improve the rna yield/purity? thank you =)

#2 namekuji

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Posted 30 June 2009 - 11:39 AM

I think by doing second extraction you would improve purity but lose on yield. Qiagen RNeasy kit is a good alternative to Trizol; you get pure (protein-free) RNA no matter what.




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