how to get rid of DNA (in TE) contamination
Posted 20 July 2009 - 01:40 PM
Posted 20 July 2009 - 11:58 PM
It seems that the aqueous (TE) phase and the chloroform phase are inversed. I can pecipitate some DNA (only 10% of the input) from the lower phase and nothing from the upper phase.
Can you (or someone else) help me ..... troubleshooting
Posted 21 July 2009 - 12:02 AM
yes it is!
Or some kind of DNA-binding spin column like you'd use to exchange buffers -- maybe the Qiagen PCR clean-up type? Is this genomic DNA?
PCR clean-up seems a good idea (although I prefer to use no kits)
or can I use a QIAamp DNA kit?