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how to get rid of DNA (in TE) contamination


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17 replies to this topic

#16 HomeBrew

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Posted 20 July 2009 - 01:40 PM

Or some kind of DNA-binding spin column like you'd use to exchange buffers -- maybe the Qiagen PCR clean-up type? Is this genomic DNA?

#17 ien

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Posted 20 July 2009 - 11:58 PM

that's what fails horribly.....
It seems that the aqueous (TE) phase and the chloroform phase are inversed. I can pecipitate some DNA (only 10% of the input) from the lower phase and nothing from the upper phase.

Can you (or someone else) help me ..... troubleshooting

#18 ien

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Posted 21 July 2009 - 12:02 AM

Or some kind of DNA-binding spin column like you'd use to exchange buffers -- maybe the Qiagen PCR clean-up type? Is this genomic DNA?

yes it is!
PCR clean-up seems a good idea (although I prefer to use no kits)
or can I use a QIAamp DNA kit?




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