Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Measuring dsRNA concentration?!


  • Please log in to reply
1 reply to this topic

#1 dcch

dcch

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 32 posts
1
Neutral

Posted 29 June 2009 - 06:21 PM

I asked my colleague to help me measure my siRNA (a type of double strand RNA) concentration after i resuspend it in siRNA buffer from pellet. The analyzer used was nanodrop. However she told me that there was no setting for dsRNA in the machine, so she measured my siRNA by setting to dsDNA.

The result is nice. However i am curious if this result is valid...can somebody help me? It is not that i don't want to measure it myself...i do not have the access to the nanodrop location :P

#2 ph1ll1ps

ph1ll1ps

    member

  • Active Members
  • Pip
  • 11 posts
0
Neutral

Posted 02 July 2009 - 06:05 AM

Ambion has some documentation about this that could be helpfull
I used it to determine my oligo concentration
http://www.ambion.co...amp;fkSubSxn=28

phillip




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.