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Unusually viscous samples


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#1 Aris

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Posted 29 June 2009 - 07:39 AM

Hi, All of a sudden my protein samples have become very viscous even after sonication. I add 2X loading buffer to the protein sample and the resulting protein sample is so viscous it gives me a very hard time when loading. I have been isolating and preparing the protein the same way and never had such viscous samples before, so this sudden problem is strange. Any help or ideas would be appreciated.

#2 genehunter

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Posted 29 June 2009 - 09:06 AM

Have you tried DNase treatment?

#3 jiajia1987

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Posted 29 June 2009 - 07:07 PM

I assume you got your protein from sonication of cells? The sample is usually viscous due to DNA as DNA is sticky. Try DNase treatment.




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