2 replies to this topic

[Aris]

Hi, All of a sudden my protein samples have become very viscous even after sonication. I add 2X loading buffer to the protein sample and the resulting protein sample is so viscous it gives me a very hard time when loading. I have been isolating and preparing the protein the same way and never had such viscous samples before, so this sudden problem is strange. Any help or ideas would be appreciated.

[genehunter]

Have you tried DNase treatment?

[jiajia1987]

I assume you got your protein from sonication of cells? The sample is usually viscous due to DNA as DNA is sticky. Try DNase treatment.