I'm using a GCMS machine and the concentration which comes out is in pmol/uL. This doesn't take into account how much sample you added and the final concentration of your sample after preparation. here is my procedure - i diluted my sample by a factor of 2 and then i took 50uL of this and added it into a tube which was then dried in a speed vac. then i added 250uL of toluene (final volume). the GCMS machine then took 1uL of this solution and produced a concentration of about 4pmol/uL. so how do i calculate what the concentration would be in pmol/uL in my original sample?
hope this makes sense.
any help would be appreciated
Edited by themoon, 29 June 2009 - 04:00 AM.