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why sometimes big or small colonies?

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16 replies to this topic

#16 DNA



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Posted 26 October 2009 - 09:39 PM

I do get big and small colonies at times. And I believe it is due to how you spread your dilution on the plates. But it is okay. It is very simply to pick small colonies. Here is one trick. Buy some toothpicks and place them in a beaker. Cover it with aluminium and send it for autoclaving. AFter that, you can always pick your tiny colonies with the toothpick and drink the toothpick into your culture tube containing LB broth and the appropriate antibiotics!

Instead of tooth picks what about the pipette tips?

#17 HomeBrew



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Posted 27 October 2009 - 03:39 AM

Instead of tooth picks what about the pipette tips?

They work, of course -- but compare the cost of a box of tips to a box of toothpicks...

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