9 replies to this topic

[Rony]

Hello everyone
am new here and i realy need help ASP
When I prepared Agarose gel 2% in TBE buffer I note that the gel disintegrate and rupture when i touch it with my  finger tp place it in the electrophoresis

what to do with this gel i cant buy new agaros i already have alot of it what to do ???


can any one help


Regards

[HomeBrew]

Welcome to the BioForums, Rony!

A 2% agarose gel in anything should be quite sturdy...  Are you sure you didn't accidently make a 0.2% gel?  A 2% gel should have 2 grams of agarose per 100 ml of buffer...

[Rony]

hi

thanks for wellcoming me  HomeBrew:D

yes i add 2gram in 100ml

befor the gell was ok until the old agarose end so i use another one i found in the lab and i face this proplem

is there any thing i can add to solidfy this bad agarose in prepration

[claritylight]

Rony, on Jun 27 2009, 12:05 PM, said:

hi

thanks for wellcoming me  HomeBrew:D

yes i add 2gram in 100ml

befor the gell was ok until the old agarose end so i use another one i found in the lab and i face this proplem

is there any thing i can add to solidfy this bad agarose in prepration

you let your 2% gel completely dry correct? i set mine to dry for 30mins as a standard and don't touch it. then i transfer it to the electrophoresis

you can try making a higher percent gel and see what is going on, like a 4%. if the 4% still is bad, i'd buy some new agarose!!

[T C]

Or chk the balance if it is calibrated and you are actually weighing 2g and not less.

Also, chk the grade of agarose, hope you are not using LMP agarose, which is fragile.

Best,
TC

[Rony]

claritylight

thanks alot i will try 4% and i will feed back

    

[Rony]

T C, on Jun 27 2009, 10:11 PM, said:

Or chk the balance if it is calibrated and you are actually weighing 2g and not less.

i did  
Also, chk the grade of agarose, hope you are not using LMP agarose, which is fragile.

what u mean by LMP agarose??

Best,

TC

Thanks   TC  

[HomeBrew]

LMP = low melting point.

Whatever agarose you're using is not behaving as expected.  Can you post some details about it from the label?

[phage434]

Agarose will not gel if the pH of the buffer is acidic.  Make sure your buffer is really a buffer.

[jiajia1987]

Also, make sure that you boil your agarose gel thoroughly before casting it. Do let us know how it goes.