hi all
Im facing problem with nondenaturing gel electrophoresis , i have to resolve 2 restriction digestion fragments that are of size 2021bp and 2109 bp ,the difference between them being 88bp. i have been trying to resolve them in 5% gel but each time i run the gel i get different result.someimes the fragments separate and sometimes they dont, again at times smiling effect is obseved. im confused and have no solution to this .
i run the gel at 6V/cm.
Is there any way to solve this problem.plz can anyone help?
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