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# TNES buffer

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### #1 zack

zack

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Posted 26 June 2009 - 09:44 AM

i need some help to prepare stock solution from above,
how to calculate 50mm Tris to 5ml volume 1 M tris.

what i know

M.W for Tris is 121.14g
1 M = 1000mM

then,

121.14g X 5000mM / 1000mM = 605.70g/L
to make 100ml, 605.70g/100 = 6.05g in 100ml

but i have on idea from calculation above to make final volume 5ml 1M Tris.
pls solve my problem

thank you

Edited by zack, 26 June 2009 - 10:01 AM.

### #2 pito

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Posted 26 June 2009 - 10:52 AM

I do not understand you really, are you sure you got the task correct? Because as I see it it says that you need 5ml of a 1M tris solution to get a 50mM solution in total (in 100ml)
ex: 8ml of a 5M NaCl sulotion is in 100ml ===> 8/100 * 5 = 0.4M

You have for each 100ml 5ml of a 1M tris solution to get a 50mM solution in total.

If you now need a 1M solution in total then you need..... ml of a 1M solution...?

ex. if you need 0.25ml of a 1M tris bottle to get a 0.1M solution in total, how much of this bottle do you need to use to get a 0.2M solution in total?

its simply x2 .... (keep in mind you need to take in this factor for the other substances to

If you don't know it, then ask it! Better to ask and look foolish to some than not ask and stay stupid.

### #3 zack

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Posted 26 June 2009 - 11:17 AM

I do not understand you really, are you sure you got the task correct? Because as I see it it says that you need 5ml of a 1M tris solution to get a 50mM solution in total (in 100ml)
ex: 8ml of a 5M NaCl sulotion is in 100ml ===> 8/100 * 5 = 0.4M

You have for each 100ml 5ml of a 1M tris solution to get a 50mM solution in total.

If you now need a 1M solution in total then you need..... ml of a 1M solution...?

ex. if you need 0.25ml of a 1M tris bottle to get a 0.1M solution in total, how much of this bottle do you need to use to get a 0.2M solution in total?

its simply x2 .... (keep in mind you need to take in this factor for the other substances to

thank.. i'm forget something

1 M tris , 5 M Nacl, 0.5 M EDTA and 10% sds is a Stock solution , so i need 50mM tris from 1 M €Tris stock solutions,

in 1 M Tris i should take 5ml of 1 M Tris to make 50mM, in TOTAL VOLUME 100ml

yes u are right..

thank for explanation

Edited by zack, 26 June 2009 - 11:19 AM.

### #4 pito

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Posted 26 June 2009 - 11:23 AM

yeah, indeed.

So I do not think you need to recalculate anything, its allready given how much you need to put in a 100ml solution to get the correct buffer.

If you don't know it, then ask it! Better to ask and look foolish to some than not ask and stay stupid.