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Acid Phenol Preparation


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#1 Electric_star

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Posted 25 June 2009 - 10:37 PM

I need to prepare acid phenol (pH 4) for RNA cleanup (after isolation, to remove DNA). We have solid phenol crystals. How do I prepare the phenol so that it is at pH 4. I know how to prepare buffered phenol pH 8 using TE. Any help would be appreciated.

#2 mdfenko

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Posted 26 June 2009 - 07:01 AM

preparation of acid phenol can be found in these protocols (found in protocols online, the parent site of this forum):

acid phenol method...

and/or

yeast rna acid phenol prep
talent does what it can
genius does what it must
i do what i get paid to do




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