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Acid Phenol Preparation

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#1 Electric_star



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Posted 25 June 2009 - 10:37 PM

I need to prepare acid phenol (pH 4) for RNA cleanup (after isolation, to remove DNA). We have solid phenol crystals. How do I prepare the phenol so that it is at pH 4. I know how to prepare buffered phenol pH 8 using TE. Any help would be appreciated.

#2 mdfenko


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Posted 26 June 2009 - 07:01 AM

preparation of acid phenol can be found in these protocols (found in protocols online, the parent site of this forum):

[url="http://www.protocol-online.org/cgi-bin/prot/view_cache.cgi?ID=869"]acid phenol method...[/url]


[url="http://labs.fhcrc.org/hahn/Methods/mol_bio_meth/acidphenol_rna.html"]yeast rna acid phenol prep[/url]
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