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uneven on gel electroforesis


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4 replies to this topic

#1 zack

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Posted 25 June 2009 - 06:39 AM

hi admin and chatter,

i had a problem which is i got uneven on the mid of gel electrophoresis, when running gel electrophoresis we can see the uneven in the middle of gel, after stain the gel, the band also follow the uneven of the loading,
why these is happen.

thank you

#2 mdfenko

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Posted 25 June 2009 - 07:04 AM

can you show us a picture of the gel? then we may be able to help you.
talent does what it can
genius does what it must
i do what i get paid to do

#3 jiajia1987

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Posted 25 June 2009 - 08:25 PM

Show us a picture of the gel and tell us what you have done in the process that led to the outcome. It will make it easier for us to understand and troubleshoot.

#4 jinx1987

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Posted 29 June 2009 - 07:23 AM

Hi,

I have encountered this problem too. It's SDS-PAGE gel. According to many protocols, I supposed to add a layer of water/butanol on top of separation gel right after pouring mixture into the apparatus. However, no matter how carefully or slow I added the water layer, the water drop would fall and mix with the separation gel, causing the uneven surface. Is there any tips on that?

Thanks,

#5 virusfan

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Posted 29 June 2009 - 08:56 AM

Hi,

I have encountered this problem too. It's SDS-PAGE gel. According to many protocols, I supposed to add a layer of water/butanol on top of separation gel right after pouring mixture into the apparatus. However, no matter how carefully or slow I added the water layer, the water drop would fall and mix with the separation gel, causing the uneven surface. Is there any tips on that?

Thanks,


I always layer with butanol and I have no such a problem.




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