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3 replies to this topic

#1 yangwenjyh

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Posted 06 August 2002 - 07:24 AM

1.why amido black can bind total transfer membrane protein?
2.ddH2O or ethanol,which one can add to the lower gel to prevent gas formation?
can you tell me,thanks

#2 bigbambu

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Posted 06 August 2002 - 03:24 PM

on gas formation just make your gel under a vaccuum then no bubbles

#3 yangwenjyh

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Posted 07 August 2002 - 07:08 AM

how to keep my gel in a vaccum condition?
i have water and alcohol only.
do i need any equipment?
thanks

#4 bigbambu

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Posted 07 August 2002 - 01:17 PM

are you making sds page gels ? triphasic? if so degas in a beaker with a vaccuum source attached then make sure the glass is TOTALLY clean and pour down one side of rig with pastuer pipette very slowly and carefully. it takes a little more time but it works for me.




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