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lysate fractionation techniques


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#1 jaknight

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Posted 24 June 2009 - 01:34 PM

I have cell lysate that I need to separate into 5 or 10 fractions. The lysate can be fractionated in any way I just need to create fractions that contain proteins at say 5-10 times normal abundance (concentration by dialysis or a similar technique will not do. I need separate fractions).

I have read about ammonium sulfate precipitation but with what I will be using the fractions for I don't like the idea of precipitating protein and would prefer an alternative.

Thanks for any help.

Edited by jaknight, 24 June 2009 - 01:36 PM.


#2 mdfenko

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Posted 25 June 2009 - 07:40 AM

you can run on gel filtration (eg sephacryl s-300, superose 6) and fractionate by size.

Edited by mdfenko, 25 June 2009 - 07:41 AM.

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