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buffer used for making brain homogenate


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#1 ujla80

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Posted 24 June 2009 - 09:09 AM

hi all

i want to know,is tris buffer pH-7.4 good for making brain homogenate ?

ujla80 :)
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#2 mdfenko

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Posted 24 June 2009 - 11:43 AM

it depends on what you are looking to isolate.

the pH and buffer are okay but you might need to add some: salt, protease inhibitors, phosphatase inhibitors, chelators and/or cations (mg, ca, mn...), non-ionic detergent (triton, nonidet, np-40, etc) or ionic detergent (sds).
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