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PKH26 staining mesenchymal stem cells


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#1 selfijini

selfijini

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Posted 24 June 2009 - 06:10 AM

Every time I use PKH26 for the staining of MSCs I lose 90% of my cells. I use 2microM for the staining of 10millions of cells with final volume 2ml.

I was thinking to reduce the final concentration. Any idea?

Thx guys

#2 asalim

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Posted 22 July 2009 - 07:35 AM

We also had similar problem and we reduced the concentration of dye to half. Now it seems ok. BTW do you know the constituent of Diluent C ?




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