3 replies to this topic

[PhD]

Hello,

I made a some 8M Urea buffer two weeks ago and since then Ive had it at room temperature. I read somewhere that u used always make it fresh and/or store it at 4C? Is this true then I cant use my big bottle of buffer

I hope someone can tell me this isnt true hehe

[PhD]

Oh and my buffer consists of Urea, Tris and NaH2PO4 in case it makes a difference

[biotechnica]

What u heard is true and will affect denaturing purification (I am assuming that is what u plan to do with the buffer)- to what extent, can be judged by doing a mini-scale purification.

Not recommended to pre-make urea containing solutions- u can try salvaging what u have if it still works in ur application.

[T C]

Urea in water releases isocyante, which will carbamylate yr proteins.  Make fresh buffer.  Read this:

http://www.ionsource...am/mono0005.htm

Best,
TC

PhD, on Jun 24 2009, 06:38 PM, said:

Hello,

I made a some 8M Urea buffer two weeks ago and since then Ive had it at room temperature. I read somewhere that u used always make it fresh and/or store it at 4C? Is this true then I cant use my big bottle of buffer

I hope someone can tell me this isnt true hehe