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Storage of 8M Urea buffer?


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#1 PhD

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Posted 24 June 2009 - 05:08 AM

Hello,

I made a some 8M Urea buffer two weeks ago and since then Ive had it at room temperature. I read somewhere that u used always make it fresh and/or store it at 4C? Is this true then I cant use my big bottle of buffer :)

I hope someone can tell me this isnt true hehe

#2 PhD

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Posted 24 June 2009 - 05:12 AM

Oh and my buffer consists of Urea, Tris and NaH2PO4 in case it makes a difference

#3 biotechnica

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Posted 24 June 2009 - 01:45 PM

What u heard is true and will affect denaturing purification (I am assuming that is what u plan to do with the buffer)- to what extent, can be judged by doing a mini-scale purification.

Not recommended to pre-make urea containing solutions- u can try salvaging what u have if it still works in ur application.

#4 T C

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Posted 27 June 2009 - 09:33 PM

Urea in water releases isocyante, which will carbamylate yr proteins. Make fresh buffer. Read this:

http://www.ionsource...am/mono0005.htm

Best,
TC

Hello,

I made a some 8M Urea buffer two weeks ago and since then Ive had it at room temperature. I read somewhere that u used always make it fresh and/or store it at 4C? Is this true then I cant use my big bottle of buffer :)

I hope someone can tell me this isnt true hehe






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