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Beta Catenin immunofluorescent staining


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#1 Bill Nye

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Posted 23 June 2009 - 04:36 PM

Hi all,

I'm trying to stain for beta catenin to visualize it's location (cytoplasmic vs nuclear) after treatment. But I keep getting punctate stains...

I am wondering if it's my fixation protocol, I used 10mins in 4% PFA.
I'm thinking of switching to cold-Methanol.

If anyone has experience with this, I would really appreciate some advice. Thanks!

B.

#2 calvin*

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Posted 23 June 2009 - 10:26 PM

I have used pre-chilled 1:1 Methanol:Acetone fixation for 5 min at -20 and 10 min room temperature, 0.2% Triton-X treatment for mammary epithelial cells for B-Catenin staining which worked fine.
"In my opinion, we don't devote nearly enough scientific research to finding a cure for jerks"-Calvin




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