Currently I grow at +37, then make a "cold shock"(put flasks on ice for 10 min), induce with 0,15 mM IPTG (instead of 1 mM) and grow overnight at +18 C. What else I can do to increase yield of soluble protein?
I have two more questions:
2) while purifying protein I have noticed that I have more impurities (presumably DnaK E.coli chaperon) than my recombinant protein. Why it can happen?
3) How does culture "owerincubation"/"overgrowth" influence yield of soluble protein?
Looking forward to your reply
P.S. mistakes corrected.
Edited by Givi, 23 June 2009 - 11:08 AM.