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Squash-preparation cytology


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#1 motus

motus

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Posted 23 June 2009 - 05:49 AM

Hello!
Iīm working with Dorosphila melanogaster and I make cytologycal preparation from brains. I have problems to squash them corectly in order to be in one layer so I can easily take photo of mitotic cells. I tried to influence them with trypsin for few minutes to break cell adherent proteins. I got cells in one layer but there were no mitotic cells. I didnīt find any protocol where people used trypsin like this. Do you have any idea where are all the mitotic cells? Or can you give me any tips and triks for cytological squash preparation?
Thanks




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