OD of bacteria at different culture volume
Posted 21 June 2009 - 03:44 AM
But when I cultured the bacteria at 1 liter culture, I seem to get slow growth after reaching OD 0.576
Will there be a difference in OD at stationary phase at different culture volume or is there something wring with my experiment? WHy is this happening?
Posted 21 June 2009 - 04:36 AM
Posted 21 June 2009 - 02:42 PM
WHile for the 1 liter I cultured it for 4 days already and its growing slow at 0.5something
Do you guys get this often?
Posted 21 June 2009 - 07:56 PM
I know I get much better growth with my bacteria when the volume of the flask is about 4x the volume ,i.e. 250ml in a 1L flask, or even more, compared say 250ml in a 500ml flask. I was told it had something to do with oxygen availability?
Posted 26 June 2009 - 05:03 AM
Posted 26 June 2009 - 01:32 PM
Posted 01 July 2009 - 01:19 PM