Posted 20 June 2009 - 04:17 AM
Posted 21 June 2009 - 05:11 PM
Posted 24 June 2009 - 06:53 AM
What do you think? Check out the Protocols pages for equilibration of phenol.
Thanks for the reply Phage
I could find answer of my first 2 question there, but not of the 3rd question. Why to saturate phenol with water for RNA extraction? What would happen if directly pure phenol is used?
Edited by ram, 24 June 2009 - 06:54 AM.
Posted 24 June 2009 - 05:59 PM
Posted 25 June 2009 - 10:51 PM
Unsaturated phenol, used to extract an aqueous sample, would become saturated -- with the water in which your RNA is dissolved...
So what? Let it saturate! Generally equal volume of phenol is used. So not all water would go into phenol. If the purpose of removing proteins and DNA from RNA is served then pure phenol also should be fine! Has anybody ever used pure phenol for RNA extraction??
Edited by ram, 25 June 2009 - 10:52 PM.
Posted 28 June 2009 - 12:02 PM
Posted 29 June 2009 - 02:59 AM
I am interested in it because it would avoid the major step of "preparation of saturated phenol"
Posted 29 June 2009 - 04:44 AM
Posted 01 July 2009 - 05:47 AM