5 replies to this topic

[rimal]

Dear all,

I have sheep antibody and I don't know the suitable conjugate, do you think Alexafluor 488 goat antirabbit is Ok? any suggestions would be appriciated.

[HomeBrew]

Why would an anti-rabbit Ab work against a sheep primary? You need some anti-sheep secondary...

[klinmed]

rimal, on Jun 19 2009, 02:07 AM, said:

Dear all,

I have sheep antibody and I don't know the suitable conjugate, do you think Alexafluor 488 goat antirabbit is Ok? any suggestions would be appriciated.

For sheep antibodies, anti-goat immunoglobulin (eg rabbit anti-goat IgG) usually work very well. You probably have a suitable reagent hanging around in your lab.

Hope this helps

[rimal]

klinmed, on Jun 20 2009, 03:34 AM, said:

rimal, on Jun 19 2009, 02:07 AM, said:

Dear all,

I have sheep antibody and I don't know the suitable conjugate, do you think Alexafluor 488 goat antirabbit is Ok? any suggestions would be appriciated.

For sheep antibodies, anti-goat immunoglobulin (eg rabbit anti-goat IgG) usually work very well. You probably have a suitable reagent hanging around in your lab.

Hope this helps

this my first time using antibodies, I have donkey anti sheep is that OK?

[klinmed]

rimal, on Jun 20 2009, 05:11 PM, said:

klinmed, on Jun 20 2009, 03:34 AM, said:

rimal, on Jun 19 2009, 02:07 AM, said:

Dear all,

I have sheep antibody and I don't know the suitable conjugate, do you think Alexafluor 488 goat antirabbit is Ok? any suggestions would be appriciated.

For sheep antibodies, anti-goat immunoglobulin (eg rabbit anti-goat IgG) usually work very well. You probably have a suitable reagent hanging around in your lab.

Hope this helps

this my first time using antibodies, I have donkey anti sheep is that OK?

Should be fine.

[DrMellotron]

Sheep and goat immunoglobulins show a high degree of homology, so an anti-goat or anti-sheep conjugate should work.
Companies that produce primary antibodies in goats or sheep normally supply suitable secondaries.