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Sheep Ab


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5 replies to this topic

#1 rimal

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Posted 18 June 2009 - 04:07 PM

Dear all,

I have sheep antibody and I don't know the suitable conjugate, do you think Alexafluor 488 goat antirabbit is Ok? any suggestions would be appriciated.

#2 HomeBrew

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Posted 18 June 2009 - 06:11 PM

Why would an anti-rabbit Ab work against a sheep primary? You need some anti-sheep secondary...

#3 klinmed

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Posted 20 June 2009 - 03:34 AM

Dear all,

I have sheep antibody and I don't know the suitable conjugate, do you think Alexafluor 488 goat antirabbit is Ok? any suggestions would be appriciated.


For sheep antibodies, anti-goat immunoglobulin (eg rabbit anti-goat IgG) usually work very well. You probably have a suitable reagent hanging around in your lab.

Hope this helps

#4 rimal

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Posted 20 June 2009 - 07:11 AM

Dear all,

I have sheep antibody and I don't know the suitable conjugate, do you think Alexafluor 488 goat antirabbit is Ok? any suggestions would be appriciated.


For sheep antibodies, anti-goat immunoglobulin (eg rabbit anti-goat IgG) usually work very well. You probably have a suitable reagent hanging around in your lab.

Hope this helps


this my first time using antibodies, I have donkey anti sheep is that OK?

#5 klinmed

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Posted 20 June 2009 - 08:30 AM

Dear all,

I have sheep antibody and I don't know the suitable conjugate, do you think Alexafluor 488 goat antirabbit is Ok? any suggestions would be appriciated.


For sheep antibodies, anti-goat immunoglobulin (eg rabbit anti-goat IgG) usually work very well. You probably have a suitable reagent hanging around in your lab.

Hope this helps


this my first time using antibodies, I have donkey anti sheep is that OK?


Should be fine.

#6 DrMellotron

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Posted 12 October 2009 - 07:21 AM

Sheep and goat immunoglobulins show a high degree of homology, so an anti-goat or anti-sheep conjugate should work.
Companies that produce primary antibodies in goats or sheep normally supply suitable secondaries.




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