Chloramphenicol resistance gene and Chemicomps E. coli (TOP10, Mach1)
Posted 18 June 2009 - 12:58 PM
Posted 19 June 2009 - 09:19 AM
I think it's more likely that your Cm resistance gene is underexpressed and isn't conferring full resistance. Did you quantify your expression levels at all?
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Posted 19 June 2009 - 03:03 PM
Horinouchi S, Weisblum B. Nucleotide sequence and functional map of pC194, a plasmid that specifies inducible chloramphenicol resistance. J Bacteriol. 1982 May;150(2):815-25. PMID: 6950931
Removal of the stem-loop allows constitutive resistance, but still significantly lower resistance than the normal E. coli cat gene expression. See http://partsregistry...Part:BBa_J70304 and the design note page for the part.