I need to know how to make a variation of the usual PCR protocol that would enable me to amplify entirely new genomic sequenceson both sides of the single known sequence. Any help would be verymuch appreciated.
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3 replies to this topic
#2
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Posted 20 May 2001 - 09:00 PM
A few solutions available for your question:1. Commercial kit like Chromosome Walker from ClonTech
2. Amplification of Gene-regulating region with single-sided specificity in PCR cloning protocol from Humana Press
3. Adaptor ligation-based PCR-mediated walkingAnalytic Biochemistry 260, 149-153,1998
#3
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Posted 21 May 2001 - 09:00 PM
Sounds like you are looking for the vectorette kit from Genosys.
#4
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Posted 31 May 2001 - 09:00 PM
You could try Tail PCR, it was developed for finding flanking regions to transposon insertions.
Efficient isolation and mapping of Arabidopsis thaliana T-DNA insert junctions by thermal asymmetric interlaced PCR. Liu YG et al, The Plant Journal (1995) 8(3) 457-463
