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A variation of the usual PCRprotocol needed


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#1 anonymous

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Posted 19 May 2001 - 09:00 PM

I need to know how to make a variation of the usual PCR protocol that would enable me to amplify entirely new genomic sequenceson both sides of the single known sequence. Any help would be verymuch appreciated.

#2 anonymous

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Posted 20 May 2001 - 09:00 PM

A few solutions available for your question:1. Commercial kit like Chromosome Walker from ClonTech

2. Amplification of Gene-regulating region with single-sided specificity in PCR cloning protocol from Humana Press

3. Adaptor ligation-based PCR-mediated walkingAnalytic Biochemistry 260, 149-153,1998


#3 anonymous

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Posted 21 May 2001 - 09:00 PM

Sounds like you are looking for the vectorette kit from Genosys.

#4 anonymous

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Posted 31 May 2001 - 09:00 PM

You could try Tail PCR, it was developed for finding flanking regions to transposon insertions.

Efficient isolation and mapping of Arabidopsis thaliana T-DNA insert junctions by thermal asymmetric interlaced PCR. Liu YG et al, The Plant Journal (1995) 8(3) 457-463





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