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Amount of genomic DNA for PCRamplication


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#1 Jason

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Posted 24 July 2001 - 09:00 PM

Can you tell me usually how much genomic DNA should be used in a 50ul PCR reaction using Taq polymerase? The DNA was obtained from a feline cell line.

#2 ragno

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Posted 26 July 2001 - 09:00 PM

Hi,

For example,with dna mouse (tail), I soak the tip pipet in dna tube and I soak in pcr mix.I have make pcr with this protocole and it's ok, as with 1 microL,2 microL but no pcr product with 3 microlThe DNA was estimated at 260nm and it was at 400ng/microL so, the amount must be about 500ng/microL.The genomic DNA must be absolutly clean for pcr testgood luke





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