I prepared my protein samples in SDS sample buffer and ran a gel using Invitrogen pre-casted gel and used a protein marker (pre-stained) on a PVDF membrane. When I did immunoblotting of my samples, I now get an actin band at 55 KDa with one antibody (Neomarkers) and at 82 KDa with another antibody (Sigma) which is of course higher than the normal mol. wt of 42KDa for actin. Both the antibodies are doing well with samples of other people. Any ideas what I am doing wrong??
Thank you in advance for your help!!
Edited by repeatcell, 18 June 2009 - 10:13 AM.