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concentration of dna


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3 replies to this topic

#1 deespike

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Posted 18 June 2009 - 09:46 AM

hi all

i am having a doubt;

what is the minimum concentration of dna that is possible to be seen in agarose gel?Is it decided from the dna ladder?

#2 eldon

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Posted 18 June 2009 - 09:52 AM

hi all

i am having a doubt;

what is the minimum concentration of dna that is possible to be seen in agarose gel?Is it decided from the dna ladder?


the minimum amount is dependent on the stain that is used. etbr DNA detection limit is about 10 ng of DNA.

#3 microgirl

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Posted 19 June 2009 - 05:09 AM

I've always been told you needed about 200 ng but I've never tested this. Generally, if my PCR worked, I can see a band if I load 5 ul from a 50 ul rxn.

#4 warsel

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Posted 20 June 2009 - 03:38 AM

Yes, I'd also say about 50-200ng for a good gel.

Edited by warsel, 20 June 2009 - 03:38 AM.





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