DNA dissolved in DMSO instead of H2O
Posted 18 June 2009 - 06:01 AM
So now I'm in a pinch, as I was planning on doing the transfection today. Does anyone have any idea how I might recover my pDNA from absolute DMSO? I was thinking of placing it in the spin-vac, but I'm interested in other ideas from the forum. Humbly,JAH
Posted 19 June 2009 - 05:40 AM
Posted 16 September 2013 - 12:48 AM
Hi, I have just made the same mistake. I dissolved my primer in DMSO instead of water :("
Thanks to Genehunter, I did what was suggetsted-- spin it down, then wash with 70% EthOH. Centrifuge again for about 30-45min. Carefully remove the EthOH and let the tube dry at room temp. Lastly, I reconstitute the tiny pellet in water. The recovered primer was used for sequencing and it worked perfectly fine
- Ameya P likes this