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[jah]

So, I had a moment of stupid this AM.  I had done a couple of midipreps that for transfection into some mammalian cells.  I did Phenol-chloroform extraction and ethanol precipitation overnight @-80'C. This morning I spun the DNA and washed with 70%EtOH, and was happy to see a nice, big pellet .   And then.......it happened.  I grabbed the eppy of DMSO to the right of the eppy of H2O, and proceeded to dissolve the pellet in DMSO instead of water.  D'oh! That's what I get for trying to do some work before coffee

So now I'm in a pinch, as I was planning on doing the transfection today.  Does anyone have any idea how I might recover my pDNA from absolute DMSO?   I was thinking of placing it in the spin-vac, but I'm interested in other ideas from the forum.  Humbly,JAH

[genehunter]

I hope DNA did  not solubilize in DMSO. You can just spin once,  get rid of it and wash with EtOH again,  then disslove it in DW or TE.