Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Electrocompetent bacteria


  • Please log in to reply
1 reply to this topic

#1 OA17

OA17

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 83 posts
3
Neutral

Posted 18 June 2009 - 04:56 AM

Hello!

I am going to buy some electrocompetent cells (bacteria) to make a DNA library. I need to have a very high transformation effiency.
Can somebody recomend me a good brand (such as Invitrogen, etc), and tell me why is it better than the others?

I already know how to prepare them in the lab, so I am not looking for a protocol, I just want some advice based on your experience for the commercial ones.

Thank you!!

#2 microgirl

microgirl

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 122 posts
0
Neutral

Posted 19 June 2009 - 05:05 AM

I've used the Invitrogen TOP10 chemically competent cells with the PCR4TOPO vector to make libraries - you get LOTS of colonies - at least several hundred per rxn if you plate the whole rxn. Maybe their electrocompetent cells are good too?

Hello!

I am going to buy some electrocompetent cells (bacteria) to make a DNA library. I need to have a very high transformation effiency.
Can somebody recomend me a good brand (such as Invitrogen, etc), and tell me why is it better than the others?

I already know how to prepare them in the lab, so I am not looking for a protocol, I just want some advice based on your experience for the commercial ones.

Thank you!!






Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.