x-ray films vs direct imaging
Posted 07 June 2010 - 10:13 PM
Posted 21 January 2011 - 02:09 AM
Posted 19 February 2011 - 06:57 AM
I've been asked to source all the equipment and reagents needed to set up western blot in the lab, starting from scratch (all we have is a Nupage electrophoresis tank). I know my preferences for transfer system, membranes, reagents.... but I'm having doubts on the developing method. In the past I've always used ECL and x-ray films, but I know more and more labs these days favour other chemiluminescent reagents and imaging as you get a better control on exposure and a better dynamic range.
So here's my question.
What method do you use to develop a western?
Please share your opinions