17 replies to this topic

[shane]

The benefit of the digital pictures is that they are effortlessly moved and you can convey out some quantitative investigation on them more effortlessly too. I acquiesce the sensitivity of the movies can occasionally be better, but its not been an topic for me. I'm attractive altered to the Digital imaging now.

[alinud]

I never develop X-ray film. Could you give me a protocol or same advice?

[Inmost sun]

Modern camera-based systems are to prefer since developing immunoblots have a longer linear range of detection; besides, classical films need a threshold level of light for darkening; a third advantage is that normally camera-based systems are offered with a professional gel documentation and analysis software

almost a doctor, on 18 June 2009 - 12:54 AM, said:

Hi guys,
I've been asked to source all the equipment and reagents needed to set up western blot in the lab, starting from scratch (all we have is a Nupage electrophoresis tank). I know my preferences for transfer system, membranes, reagents.... but I'm having doubts on the developing method. In the past I've always used ECL and x-ray films, but I know more and more labs these days favour other chemiluminescent reagents and imaging as you get a better control on exposure and a better dynamic range.

So here's my question.

What method do you use to develop a western?


Please share your opinions

Thanks!