17 replies to this topic

[shane]

The benefit of the digital pictures is that they are effortlessly moved and you can convey out some quantitative investigation on them more effortlessly too. I acquiesce the sensitivity of the movies can occasionally be better, but its not been an topic for me. I'm attractive altered to the Digital imaging now.

[alinud]

I never develop X-ray film. Could you give me a protocol or same advice?

[Inmost sun]

Modern camera-based systems are to prefer since developing immunoblots have a longer linear range of detection; besides, classical films need a threshold level of light for darkening; a third advantage is that normally camera-based systems are offered with a professional gel documentation and analysis software

Hi guys,
I've been asked to source all the equipment and reagents needed to set up western blot in the lab, starting from scratch (all we have is a Nupage electrophoresis tank). I know my preferences for transfer system, membranes, reagents.... but I'm having doubts on the developing method. In the past I've always used ECL and x-ray films, but I know more and more labs these days favour other chemiluminescent reagents and imaging as you get a better control on exposure and a better dynamic range.

So here's my question.

What method do you use to develop a western?


Please share your opinions

Thanks!