I've been asked to source all the equipment and reagents needed to set up western blot in the lab, starting from scratch (all we have is a Nupage electrophoresis tank). I know my preferences for transfer system, membranes, reagents.... but I'm having doubts on the developing method. In the past I've always used ECL and x-ray films, but I know more and more labs these days favour other chemiluminescent reagents and imaging as you get a better control on exposure and a better dynamic range.
So here's my question.
What method do you use to develop a western?

Please share your opinions
Thanks!
