Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Lysyl Oxidase


  • Please log in to reply
No replies to this topic

#1 Aris

Aris

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 67 posts
0
Neutral

Posted 17 June 2009 - 07:05 PM

Hi all,

has anybody had experience with the Lysyl Oxidase enzyme aka LOX? It seems very hard to visualise with western blot. Moreover there is a great dispute about the primary antibodies currently available that they are not sensitive enough, non sprecific binding etc. I am using PVDF and a standard protocol that i know that it works. There are a few publications out there that show good western blots of LOX but they are oly a few and also they say that LOX is notoriously hard to visualise with western blots.
Any background info on that? Any primaries that you know that they work good?? Any tips on reducing non specific binding?




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.