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Establishing a new cell line
1 reply to this topic
Posted 17 June 2009 - 12:57 PM
We are culturing a tumor and i'm wondering how many passages out do you need to go before really believing you've established a "cell line"?
Posted 17 June 2009 - 04:17 PM
Out to a point beyond the normal Hayflick limit for a particular cell type would be one definition, however there are quite a few cell lines out there that are not truly immortal.