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Establishing a new cell line


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#1 MPdev

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Posted 17 June 2009 - 12:57 PM

We are culturing a tumor and i'm wondering how many passages out do you need to go before really believing you've established a "cell line"?

Thanks!

#2 bob1

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Posted 17 June 2009 - 04:17 PM

Out to a point beyond the normal Hayflick limit for a particular cell type would be one definition, however there are quite a few cell lines out there that are not truly immortal.




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