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Centrifugation of epithelial cells


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#1 floyd78

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Posted 17 June 2009 - 03:02 AM

Can fixed angle centrifuge rotors be used to spin down cultured epithelial cells? If so are any changes in normal speeds/times necessary? I currently use 220g for 4 mins in a swinging bucket system but will shortly be moving labs so will only have access to a fixed angle system temporarily.

Thanks

#2 bob1

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Posted 17 June 2009 - 04:20 PM

They should be OK, you might want to slow the acceleration (dw - delta omega) a bit though

#3 floyd78

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Posted 17 June 2009 - 10:52 PM

Thanks very much :lol:




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