how to know that our sequence hit the gene not the primer?im so confused....
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HomeBrew
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Posted 17 June 2009 - 03:49 AM
Wlecome to the BioForum, hazrina!
I'm not sure what you're asking... Did you obtain some sequence data using a sequencing primer? If so, the sequence will begin (theoretically) at a point one base 3' to where the primer binds. You usually can't read the first 10-20 bases of the sequence, so the sequence proper would begin some short distance away from the 3' end of the primer.
If you re-ask your question with more detail about what you did and what you're confused about, I'm sure we can be of more help...
I'm not sure what you're asking... Did you obtain some sequence data using a sequencing primer? If so, the sequence will begin (theoretically) at a point one base 3' to where the primer binds. You usually can't read the first 10-20 bases of the sequence, so the sequence proper would begin some short distance away from the 3' end of the primer.
If you re-ask your question with more detail about what you did and what you're confused about, I'm sure we can be of more help...
