Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
- - - - -

sequencing result for primer designing

  • Please log in to reply
1 reply to this topic

#1 hazrina



  • Active Members
  • Pip
  • 11 posts

Posted 17 June 2009 - 01:19 AM

how to know that our sequence hit the gene not the primer?im so confused....

#2 HomeBrew



  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 930 posts

Posted 17 June 2009 - 03:49 AM

Wlecome to the BioForum, hazrina!

I'm not sure what you're asking... Did you obtain some sequence data using a sequencing primer? If so, the sequence will begin (theoretically) at a point one base 3' to where the primer binds. You usually can't read the first 10-20 bases of the sequence, so the sequence proper would begin some short distance away from the 3' end of the primer.

If you re-ask your question with more detail about what you did and what you're confused about, I'm sure we can be of more help...

Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.