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need correction on dilution


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3 replies to this topic

#1 biogirl1230

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Posted 15 June 2009 - 05:29 PM

1. I have 1000ug/ml of Sample A. I need to bring it to 200ul final volume and 10ug final concentration.

C1V1=C2V2
C1 = 1000ug/ml
V1 = x
C2 = 10ug/0.2ml = 50ug/ml
V2 = 200ul

V1 = (50*200) / 1000
V1 = 10ul

10ul of stock + 190ul water = 200ul of 10ug of Sample A


2. I have 2000ng/ul of Sample B. I need to bring it to 200ul final volume and 10ug final concentration.

C1 = 2000ng/ul = 2000ug/ml
V1 = x
C2 = 10ug/0.2ml = 50ug/ml
V2 = 200ul

V1 = (50*200) / 2000
V1 = 5ul

5ul of stock + 195ul water = 200ul of 10ug of Sample B

#2 pcrman

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Posted 15 June 2009 - 08:14 PM

Your calculations are correct

#3 Rsm

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Posted 15 June 2009 - 08:16 PM

10ug is not a concentration, it is an amount.
If you need 10ug total, your calculation is correct.
I got soul, but I'm not a soldier

#4 Rsm

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Posted 15 June 2009 - 08:17 PM

Your calculations are correct

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