2 replies to this topic

[Pluie]

Dear All,


Attached is a dissociation curve of a real-time PCR ran on a stratagene machine MX3000. Does it look alright, would you trust results from such a curve? The sequence amplified is a Beta-actin sequence.

As you can see only the signal H4 has a secondary peak higher than the product peak.




Thanks,

P.

[gfischer]

I'd be a little concerned about H4.  Was it a control reaction or a sample?

[Pluie]

H4 is  a sample. Negative samples were negative. The four highest peaks corresponds to the genomic control at 4 different concentrations.

If we ignore H4, what about the rest of the curve, is it acceptable to use the Ct values?

Edited by Pluie, 15 June 2009 - 10:32 AM.