Judging a dissociation curve
Posted 15 June 2009 - 09:25 AM
Attached is a dissociation curve of a real-time PCR ran on a stratagene machine MX3000. Does it look alright, would you trust results from such a curve? The sequence amplified is a Beta-actin sequence.
As you can see only the signal H4 has a secondary peak higher than the product peak.
Posted 15 June 2009 - 10:19 AM
then heaven will be yours, before you meet your end
Posted 15 June 2009 - 10:27 AM
If we ignore H4, what about the rest of the curve, is it acceptable to use the Ct values?
Edited by Pluie, 15 June 2009 - 10:32 AM.