Knock-out MEFs and WT MEFs
Posted 15 June 2009 - 09:05 AM
I want to see if there's a growth defect with my virus grown in knock-out MEFs versus WT MEFs. Since the MEFs came from different mice, if I do see a growth defect, should I be concerned that it may not be due to the knock-out? Thanks!
Posted 15 June 2009 - 04:23 PM
Posted 15 June 2009 - 07:50 PM
If the strain is different, couldn't you just make some MEF from the same strain as your knock-out? I think that would be the best way to proceed.