Hello,
I want to see if there's a growth defect with my virus grown in knock-out MEFs versus WT MEFs. Since the MEFs came from different mice, if I do see a growth defect, should I be concerned that it may not be due to the knock-out? Thanks!
Submit your paper to J Biol Methods today!
2 replies to this topic
#2
bob1
-
- Global Moderators
-
- 6,387 posts
Thelymitra pulchella
512
Excellent
Excellent
Posted 15 June 2009 - 04:23 PM
The standard way to do this experiment would be to have non-infected controls for both the WT and KO MEFs as well as the infected treatments, this should tell you if you have comparable growth from both the WT and KO.
#3
leelee
-
- Active Members
-
- 652 posts
Veteran
53
Excellent
Excellent
Posted 15 June 2009 - 07:50 PM
Do you mean the MEF are a different genetic background strain?? Or is your knock-out made from the WT strain??
If the strain is different, couldn't you just make some MEF from the same strain as your knock-out? I think that would be the best way to proceed.
If the strain is different, couldn't you just make some MEF from the same strain as your knock-out? I think that would be the best way to proceed.
