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Knock-out MEFs and WT MEFs


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#1 BottegaVeneta

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Posted 15 June 2009 - 09:05 AM

Hello,

I want to see if there's a growth defect with my virus grown in knock-out MEFs versus WT MEFs. Since the MEFs came from different mice, if I do see a growth defect, should I be concerned that it may not be due to the knock-out? Thanks!

#2 bob1

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Posted 15 June 2009 - 04:23 PM

The standard way to do this experiment would be to have non-infected controls for both the WT and KO MEFs as well as the infected treatments, this should tell you if you have comparable growth from both the WT and KO.

#3 leelee

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Posted 15 June 2009 - 07:50 PM

Do you mean the MEF are a different genetic background strain?? Or is your knock-out made from the WT strain??

If the strain is different, couldn't you just make some MEF from the same strain as your knock-out? I think that would be the best way to proceed.




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